Sensing of Lung Cancer using Surface Potential
A label-free and high-sensitive sensing method of lung cancer mutations using surface potential and a gold
nanoparticle (AuNP) is developed. The detection is based on the evaluation of the surface potential of each AuNP. When
AuNPs are modified with probe DNA (AuNP–pDNA), the surface potential is shifted towards the negative potential due to
the negatively charged DNA backbone. When AuNP–pDNA captures target mutant DNA through DNA hybridization, an
additional surface potential shift occurs. The platform is able to sense lung cancer mutant DNA with a limit of detection
(LOD) of 3.3 pM.
Keywords- Lung Cancer; Surface Potential; Gold Nanoparticle; Probe DNA