Paper Title :Proteomics of Broiler’s Liver
Author :Muhamad Shafiq Abu Hassan, Muhammad Syazwan Saidin, Nurul Ashima Hamdan, Shafida Abd Hamid, Noraslinda Muhamad Bunnori
Article Citation :Muhamad Shafiq Abu Hassan ,Muhammad Syazwan Saidin ,Nurul Ashima Hamdan ,Shafida Abd Hamid ,Noraslinda Muhamad Bunnori ,
(2018 ) " Proteomics of Broiler’s Liver " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 8-17,
Volume-6, Issue-2
Abstract : In this study, the differences in protein expression from chicken liver between commercial broilers (CB) and indigenous
chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted
adjacent to consumer experience in order to find out the differences of the protein profile in liver tissues between the two
popular types of chickens that have been offered to the Malaysians consumer for over 50 years. In 2D-PAGE, six spots were
successfully identified differentially expressed in term of intensity. Those proteins are phosphoenolpyruvate carboxykinase
(PEPCK) (2 spots), delta-1-pyrolline-5-carboxylate (P5CDH), soluble epoxide hydrolase (sEH), heat shock protein 90
(HSP90) and γ-actin. PEPCK and P5CDH enzymes were expressed higher in CB compared to IC, suggesting the utilization
of alternative metabolic pathways for broilers’ energy metabolism. The sEH expression was found higher in CB compared to
IC probably due to the process of xenobiotic metabolic activity to eliminate harmful epoxide inside the CB’s liver. HSP90
was found less in CB compared to IC, which may indicate the lack of regulation of heat stress response in CB. The higher
expression of actin in IC liver suggested a good form of structure integrity in IC liver compared to CB, which may happen
due to the age differences.Most of the differentially expressed proteins are involved in energy metabolism, stress regulation,
and cellular structure.These differences could arise from many variables involved which may include breeds, age, feed
regimen, drug treatment and rearing environment.
Keywords - Chicken, Broilers, Indigenous, Proteomics, 2D-Electrophoresis, Liver
Type : Research paper
Published : Volume-6, Issue-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-11994
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Copyright: © Institute of Research and Journals
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Published on 2018-06-29 |
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