Paper Title :Production of Biopolymer from Locally Isolated Bacteria in Kurdistan Region-Erbil/Iraq
Author :Zhyan Othman Hamasadek, Abdul Jabbar O. Qoja, Zardasht Sh. Rasool
Article Citation :Zhyan Othman Hamasadek ,Abdul Jabbar O. Qoja ,Zardasht Sh. Rasool ,
(2023 ) " Production of Biopolymer from Locally Isolated Bacteria in Kurdistan Region-Erbil/Iraq " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 106-109,
Volume-11,Issue-2
Abstract : The study was aimed explore possibility of isolate microorganisms which isolated from different sources to
produce a biopolymer. Bioplastics are considered as environmentally friendly will complete biodegradable after disposal.
The collection of 130 isolates bacteria from different samples from Erbil city and neighbouring regions during November
2014 to February 2015, and screening for poly 3-hydroxybutyrate (P3HB) production, only 20 bacterial isolates have the
ability for the P3HB production. Accordant to quantity and quality,one of isolates was selected to further study.According to
the result of polymer chain reaction (PCR) the G1 isolate identified as Citrobacterbraakii, isolate.The quantity of product
depended on cell dry weight percentage was ranged from (3.939 - 98.34 %). Also, estimation of (P3HB) concentration by
crotonic acid assay (standard curve) the concentration of P3HB was ranged from (0.03394- 0.00868) g/l.Nuclear magnetic
resonance (NMR) spectrum (1HNMR) technique was performed for obtaining information about the number and
arrangement of hydrogen atoms in P3HB by their chemical shift data.
It can be concludedthatthere are several strains of bacteriaable to produce biopolymer. More of these isolates were found
from swage water and soil mountain sources. New biopolymer produced isolate was identified as (Citrobacterbraakii).
Keywords - Biopolymer, Poly 3-hydroxybutyrate(P3HB),1HNMR , Nile blue A
Type : Research paper
Published : Volume-11,Issue-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-19967
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Published on 2023-10-27 |
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